Keller kiliani test for steroids

Powdered plant materials ----------------- extracted with chloroform ------------------ sonicated for 10minzz ------------leave at room temperature for 1 hour ----------------filter through paper filter and wash with chloroform ---------------- evaporate to dryness --------------add 5ml chloroform and 2ml of sulphuric acid -------------- mix thoroughly -------------- remove chloroform and adjust the pH with ammonium hydroxide ------------------ extract alkaloids with 2 ml of chloroform -------------- filter by addition of anhydrous sodium sulphate -------------------- evaporate it by adding with sodium hydroxide.

Keller kiliani test for steroids

keller kiliani test for steroids

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